Download PDF by Shan S. Wong,David M. Jameson: Chemistry of Protein and Nucleic Acid Cross-Linking and

By Shan S. Wong,David M. Jameson

Since the booklet of the 1st variation of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, particularly multifunctional cross-linkers, were built and synthesized. The of entirety of the human genome venture has opened a brand new sector for learning nucleic acid and protein interactions utilizing nucleic acid cross-linking reagents, and advances have additionally been made within the zone of biosensors and microarray biochips for the detection and research of genes, proteins, and carbohydrates. moreover, advancements in actual options with remarkable sensitivity and determination have facilitated the research of cross-linked items.

Updated to mirror the advances of the twenty first century, this ebook offers:

  • An evaluate of the chemical rules underlying the approaches of cross-linking and conjugation

  • A thorough checklist of cross-linking reagents released within the literature because the first variation, overlaying monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers

  • Reviews of using those reagents in learning protein tertiary constructions, geometric preparations of subunits inside of advanced proteins and nucleic acids, near-neighbor research, protein-to-protein or ligand–receptor interactions, and conformational alterations of biomolecules

  • Discusses the appliance of immunoconjugation for immunoassays, immunotoxins for exact remedy, microarray know-how for research of varied biomolecules, and reliable kingdom chemistry for immobilizations

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Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation, Second Edition by Shan S. Wong,David M. Jameson

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